Anti-undefined Antibody ALK,1504 Unconjugated BHA10208067

Mfr.No.	238-MSM4-P1ABX
Alternative Names	ALK Tyrosine Kinase Receptor, ALK/NPM1 fusion gene, Anaplastic lymphoma kinase Ki1, Anaplastic Lymphoma Kinase p80, anaplastic lymphoma receptor tyrosine kinase, CD246, mutant anaplastic lymphoma kinase, NBLST3, Tcrz, TFG/ALK
Categories	Primary Antibodies
Cellular Localization	Nuclear, Cytoplasmic
Chromosome Location	2p23
Clonality	monoclonal
Description	The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.
Host	Mouse
Immunogen	Recombinant human ALK protein fragment (around aa 200-335) (exact sequence is proprietary)
Isotype	IgG2b Kappa
Positive Control	Anaplastic Large Cell Lymphoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	Q9UM73
Gene Id	238
Clone No	1504, ALK
Symbol	ALK
Weight	80kDa (hybrid); 200kDa (wild type)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

1-2μg/ml for 30 minutes at RT