Anti-Transgelin (SM22-alpha) Antibody BHA10205123

Mfr.No.	6876-MSM1-P0
Alternative Names	2kDa actin-binding protein ; Human 22kDa smooth muscle protein ; Protein WS3-10 ; SMCC ; Smooth muscle protein 22-alpha (SM22-alpha) ; TAGLN ; TAGLN1 ; transgelin ; Transgelin variant 2 ; WS3-10 Human Gene SymbolTAGLNHu Chromosome Location11q23.2
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	11q23.2
Clonality	Monoclonal
Description	This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
Host	Mouse
Immunogen	Recombinant full-length human transgelin (TAGLN) protein.
Isotype	IgG1 Kappa
Positive Control	U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.
Reactivity	Human, Cow, Pig, Rabbit, Mouse
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q01995
Uniprot	410977
Gene Id	6876

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution