Anti-Topoisomerase II alpha Antibody BHA10205428

Mfr.No.	7153-MSM1-P1ABX
Alternative Names	ATP hydrolyzing DNA topoisomerase II alfa ; DNA gyrase ; DNA topoisomerase (ATP hydrolyzing) ; DNA topoisomerase 2 alpha ; DNA topoisomerase II 170kD ; DNA topoisomerase II alpha ; DNA Topoisomerase2 ; TOP2A ; Topoisomerase DNA II alpha 170kDa ; TP2A
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	17q21.2
Clonality	Monoclonal
Description	It recognizes a 170kDa protein, which is identified as topoisomerase II.  shows no cross-reaction with Topoisomerase II or I. Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II is also implicated in drug resistance of tumor cells.
Host	Mouse
Immunogen	Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
Isotype	IgG2b Kappa
Positive Control	HeLa cells or Tonsil
Reactivity	Human, Mouse
Recombinant	FALSE
Regulatory	RUO

Swissprot	P11388
Uniprot	156346
Gene Id	7153

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution