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Home / Primary Antibodies / IHC Antibodies

Anti-Nuclear Membrane Marker Antibody (AE-5) BHA10207504

SKU : BHA10207504

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NeoBiotechnologies, Inc.

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Mfr.No.	MSM2-867-P0
Antigen	Nuclear Membrane Marker
Categories	Primary Antibodies ; Tag & Cell Markers
Cellular Localization	Nuclear membrane
Clonality	monoclonal
Description	This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present on the nuclear envelope to facilitate and regulate the exchange of materials (for example, proteins and RNA) between the nucleus and the cytoplasm. The space between the two membranes that make up the nuclear envelope is called the perinuclear space (also called the perinuclear cisterna), and is usually about 20 - 40 nm wide. Each of the two membranes is composed of a lipid bilayer. The outer membrane is continuous with the rough endoplasmic reticulum. The inner membrane is erected upon the nuclear lamina, a network of intermediate filaments made of lamin, that plays a role in mitosis and meiosis. The type of lamins present are A, B1, B2, and C. The nuclear envelope may also play a role in the disposition of chromatin inside the nucleus. The lamina acts as a site of attachment for chromosomes. It also acts like a shield for the nucleus. During prophase in mitosis, the chromatids begin condensing to form chromosomes, and the nuclear envelope begins to disintegrate. During metaphase, the nuclear envelope is completely disintegrated, and the chromosomes can be pulled apart as chromatids by the spindle fibers.
Host	Mouse
Immunogen	Nuclei of myeloid leukemia biopsy cells
Positive Control	Tonsil
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Not Known
Uniprot	Not Known
Gene Id	Not Known
Clone No	AE-5
Symbol	Not Known
Weight	Not Known

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

IF, IHC(fro)

Description

Optimal dilution for a specific application should be determined., IF ; IHC(fro)

Dilution

IF	0.5-1.0μg/ml
IHC(fro)	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207504	MSM2-867-P0	20µg	$204.67		Add to Cart
BHA10207504	MSM2-867-P0	100µg	$393.20		Add to Cart

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