Anti-Neurofilament (H+L) Antibody BHA10207496

Mfr.No.	MSM2-756-P1ABX
Alternative Names	NEFH ; Neurofilament H ; Neurofilament Heavy Polypeptide 200kDa ; Neurofilament Triplet H Protein ; NF-H ; NF200. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	22q12.2 (NEFH) ; 8p21.2 (NEFL)
Clonality	Monoclonal
Description	This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Host	Mouse
Immunogen	Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)
Positive Control	Brain, Neuroblastoma
Reactivity	Human, Mouse, Rat, Chicken, Pig
Recombinant	FALSE
Regulatory	RUO

Swissprot	P12036; P07196
Uniprot	198760; 521461
Gene Id	4744;4747

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

IF, FACS, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., IF ; FACS ; IHC-F

Dilution

IF	1:25-1:50
FACS	0.5-1μg/million cells in 0.1ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207496	MSM2-756-P1ABX	100µg	$393.20		Add to Cart

Anti-Neurofilament (H+L) Antibody BHA10207496

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