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Home / Primary Antibodies / Phospho Antibodies

Anti-MAPK1/MAPK3 Antibody BHA11300102

SKU : BHA11300102

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Mfr.No.	AP0490
Alternative Names	Mitogen-activated protein kinase 3 ; MAP kinase 3 ; MAPK 3 ; ERT2 ; Extracellular signal-regulated kinase 1 ; ERK-1 ; Insulin-stimulated MAP2 kinase ; MAP kinase isoform p44 ; p44-MAPK ; Microtubule-associated protein 2 kinase ; p44-ERK1 ; MAPK3 ; ERK1 ; PRKM3 ; Mitogen-activated protein kinase 1 ; MAP kinase 1 ; MAPK 1 ; ERT1 ; Extracellular signal-regulated kinase 2 ; ERK-2 ; MAP kinase isoform p42 ; p42-MAPK ; Mitogen-activated protein kinase 2 ; MAP kinase 2 ; MAPK 2 ; MAPK1 ; ERK2 ; PRKM1 ; PRKM2
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Host	Rabbit
Immunogen	Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.
Modification	phospho-Y204
Reactivity	Human, Mouse, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P27361/P28482
Uniprot	P27361/P28482
Weight	42,44 kDa

Description	Product: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. Specificity: p-ERK1/2 (Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at Tyr204, and ERK2 protein when phosphorylated at Tyr187.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Applications

WB, IHC, IF

Description

WB ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:200
IF	1:50-1:200

Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA11300102	AP0490	50µl	$199.33		Add to Cart
BHA11300102	AP0490	100µl	$314.89		Add to Cart

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