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Home / Primary Antibodies / Monoclonal Antibodies

Anti-IL-2RA (CD25) Antagonist Antibody

SKU : BHA18200027

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BPS Bioscience

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Mfr.No.	101593
Description	Recombinant monoclonal anti-CD25 (Basiliximab) which recognizes interleukin-2 receptor alpha subunit (IL-2RA). This affinity purified antibody has been tested for specific binding to purified human IL2RA protein (BPS Bioscience #101203) in a colorimetric ELISA binding assay. assay conditions: Functional validation: The antibody was validated by measuring Anti-IL-2RA binding to IL2RA antigen in ELISA assay. The IL2RA protein (BPS Bioscience #101203) was coated onto a 96-well plate oveight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified Anti-IL-2RA Antibody (BPS Bioscience #101593), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	HEK293
Instructions for use	Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Molecular Weight	Heavy Chain: 50 kDa; Light Chain: 23 kDa + glycans
Species	Human
Synonyms	Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
Note	Avoid freeze/thaw cycles

Uniprot	P01589

Format	Aqueous buffer solution
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200027	101593	50 ug	$308		Add to Cart

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