This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Host
Mouse
Immunogen
Human EGFR purified from A431 cells
Isotype
IgG2a Kappa
Positive Control
A431 cells. Placenta, Breast, Colon or Bladder cancer
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P00533
Uniprot
488293
Gene Id
1956
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C) ,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
1-2μg/ml
IHC-F
2-4μg/ml for 30 minutes at RT
Reviews of Anti-EGFR (Epidermal Growth Factor Receptor) Antibody BHA10200897