It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Host
Mouse
Immunogen
Cytoskeletal preparation from HeLa cells
Positive Control
HepG2 or A431 cells. Skin or Squamous cell carcinoma
Reactivity
Human, Pig, Mouse, Rat, Hamster, Cow, Dog, Sheep, ferret
Recombinant
FALSE
Regulatory
RUO
Swissprot
P13647 (CK5); P05787 (CK8)
Uniprot
433845 (CK5); 533782 & 708445 (CK8)
Gene Id
3852 (CK5);3856 (CK8)
Buffer
0.5 ml at 200g/ml of antibody purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1.0μg/ml
WB
0.5-1.0μg/ml
IHC-F
0.5-1.0μg/ml for 30 min at RT
Reviews of Anti-Cytokeratin 5/8 Antibody BHA10207267