Anti-Cytokeratin 10/13 Antibody BHA10207253

Mfr.No.	MSM11-606-P1ABX
Alternative Names	BCIE ; BIE ; EHK ; Keratin Type I Cytoskeletal 10 ; KRT10 ; CK13 ; K13 ; keratin type I cytoskeletal 13 ; Keratin-13 ; Krt1-13
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	17q21.2
Clonality	Monoclonal
Description	This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Host	Mouse
Immunogen	Cytoskeletal preparation extracted from human ectocervical epithelium
Positive Control	A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
Reactivity	Human, Cat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P13645; P13646
Uniprot	99936; 654550
Gene Id	3858;3860

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
WB	0.25-0.5μg/ml
IHC-F	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207253	MSM11-606-P1ABX	100µg	$393.20		Add to Cart

Anti-Cytokeratin 10/13 Antibody BHA10207253

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