Charcot Marie Tooth neuropathy X linked ; CMTX ; CMTX1 ; Connexin-32 ; Cx32 ; GAP junction 28kDa liver protein ; Gap junction beta-1 protein ; Gap junction protein beta 1 32kD ; GJB1
Categories
Primary Antibodies
Cellular Localization
Cell Surface, Cytoplasmic
Chromosome Location
Xq13.1
Clonality
Monoclonal
Description
This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
Host
Mouse
Immunogen
Recombinant human GJB1 protein
Isotype
IgG
Positive Control
MCF-7 Cells. Liver, Kidney, Stomach or Tonsil
Reactivity
Human, Mouse, Rat
Recombinant
FALSE
Regulatory
RUO
Swissprot
P08034
Uniprot
333303
Gene Id
2705
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
IF, FACS, WB, IHC(fro)
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., IF ; FACS ; WB ; IHC(fro)
Dilution
IF
1-2μg/ml
FACS
0.5-1μg/million cells
WB
0.5-1μg/ml
IHC(fro)
1-2μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-Connexin 32 (Gap Junction Protein) Antibody BHA10201479