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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Claudin-18 Isoform 2 IgG Antibody, Biotin-Labeled

SKU : BHA18200080

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BPS Bioscience

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Mfr.No.	101565-1
Description	Biotinylated anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 antibody is a purified recombinant antibody that recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding to purified human CLDN18.2 protein. assay conditions: Functional validation: The antibody was validated by measuring anti-CLDN18.2 binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate oveight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-beta-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified biotinylated anti-CLDN18.2 antibody (BPS Bioscience #101565), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.Flow cytometry validation: One million cells were stained with 1 ug of biotinylated anti-CLDN18.2 IgG for 30 minutes on ice, washed three times, stained with Streptavidin PE Conjugate (ThermoFisher #12-4317-87) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	Heavy chain (HC) and Light chain (LC) co-expressed in HEK293
Isotype	IgG1
Molecular Weight	Total: 150 kDa; Light Chain: 24 kDa; Heavy Chain: 51 kDa
Species	Human
Synonyms	CLDN18, UNQ778, PRO1572
Note	Avoid freeze/thaw cycles.

Uniprot	P56856

Format	Aqueous buffer solution
Purification	Protein A affinity
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200080	101565-1	50 ug	$390.50		Add to Cart

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