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Home / Primary Antibodies / Monoclonal Antibodies

Anti-CD20 IgG Antibody, Biotin-labeled

SKU : BHA18200068

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BPS Bioscience

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Mfr.No.	101207-1
Description	Biotinylated anti-CD20 IgG antibody is a purified recombinant antibody which recognizes human CD20 antigen. This antibody has been tested for specific binding to purified human CD20 protein. assay conditions: Functional validation: The antibody was validated by ELISA using a purified CD20 antigen. The CD20 protein was coated onto a 96-well plate oveight at 4°C (50 ul/well at a concentration of 4 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified biotinylated anti-CD20 antibody (BPS Bioscience #101207), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	Heavy chain (HC) and Light chain (LC) co-expressed in HEK293
Molecular Weight	Heavy Chain: 53 kDa; Light Chain 23 kDa
Species	Human
Note	Avoid freeze/thaw cycles.

Format	Aqueous buffer solution
Purification	Protein A affinity
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200068	101207-1	50 ug	$368.50		Add to Cart

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