This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.
Host
Mouse
Immunogen
Recombinant chicken ?-catenin
Isotype
IgG1 Kappa
Positive Control
HeLa or MCF-7 cells. Breast carcinoma
Reactivity
Human, Cow, Dog, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P35222
Uniprot
476018
Gene Id
1499
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA at 1.0mg/ml
Applications
FACS, IF, WB, IHC(af)
Description
Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC(af)
Dilution
FACS
0.5-1μg/million cells
IF
1-2μg/ml
WB
0.5-1μg/ml
IHC(af)
Reviews of Anti-Catenin, beta (p120) Antibody BHA10200658