Anti-Blood Group Antigen A (CD173) Antibody BHA10201603

Mfr.No.	28-MSM4-P1ABX
Alternative Names	A transferase ; ABO ; B transferase ; CD173 ; Fucosylglycoprotein 3-alpha-galactosyltransferase ; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase ; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase ; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase ; Histo-blood group A transferase ; Histo-blood group B transferase
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	9q34.2
Clonality	monoclonal
Description	This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
Host	Mouse
Immunogen	Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A
Isotype	IgM Kappa
Positive Control	KG1 cells or human colorectal carcinoma tissues.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P16442
Uniprot	654423
Gene Id	28

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

AGG, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., AGG ; IF ; IHC-F

Dilution

AGG
IF	0.5-1μg/ml
IHC-F	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201603	28-MSM4-P1ABX	100µg	$393.20		Add to Cart

Anti-Blood Group Antigen A (CD173) Antibody BHA10201603

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