Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
Host
Mouse
Immunogen
Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)
Isotype
IgG2b Kappa
Positive Control
HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
Q99541
Uniprot
3416
Gene Id
123
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., ELISA ; IF ; IHC-F
Dilution
ELISA
For coating, order Ab without BSA
IF
0.5-1μg/ml
IHC-F
2-4μg/ml for 30 minutes at RT
Reviews of Anti-Adipophilin / Perilipin-2 Antibody BHA10200532