β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.
Categories
Primary Antibodies ; Control Antibodies
Clonality
monoclonal
Description
Featured Anti-β-Actin Mouse Monoclonal Antibody (1C7), Biotin conjugated specially designed for your WB or IHC-P analysis.
Formulation
Liquid solution
Host
Mouse(Mu)
Immunogen
Synthetic Peptide
Isotype
IgG1
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity
Chicken, Hamster, Insect, Monkey, Rabbit, Human, Mouse, Dog, Rat
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Storage Buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IHC-P (1:200).
Datasheet
Reviews of Anti-β-Actin Mouse Monoclonal Antibody (1C7), Biotin Conjugated