The protein encoded by PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1) belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Clonality
Monoclonal
Description
Mouse Anti-AMPK alpha1 Monoclonal Antibody
Formulation
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen, Liquid solution
Host
Mouse
Immunogen
Synthetic Peptide
Immunogen Region
Synthetic Peptide
Isotype
Mouse IgG1
Molecular Weight
63 KD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity
Human, Mouse, Rat
Tag
AMPK alpha1
Note
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:50-1:100).
Accession Number
Q13131
Gene Id
5562, 5562
Concentration
1 mg/ml, 1 mg/ml
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen, The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Storage Buffer
PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.