Rabbit Anti-Human Akt2 (PKB beta) Polyclonal

Mfr.No.	SPC-148, SPC-148F, SPC-148F-A390, SPC-148F-A488, SPC-148F-A594, SPC-148F-BI, SPC-148F-FITC
Immunogen Species	Human
Alternative Names	AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus, Cell Membrane
Certificate Of Analysis	A 1:3000 dilution of SPC-148 was sufficient for detection of PKBbeta in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Clonality	Polyclonal
Description	Rabbit Anti-Human Akt2 (PKB beta) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH, Homo Sapiens (Human)
Immunogen Region	A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH
Launch Date	2007/06/01
Net Weight (g)	0.4
Reactivity	Guinea Pig, African clawed frog, Dog, Pig, Rat, Sheep, Mouse, Human, Monkey, Bovine, Rabbit, Hamster, Chicken
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Human

Swissprot	P31751
Accession Number	NP_001617.1
Gene Id	208
Target	AKT2
Research Area	Cell Signaling, Post-translational Modifications, Phosphorylation

Buffer	TBS, 50% glycerol, 0.01% sodium azide
Concentration	0.25 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~65kDa. Does not react with PKBalpha (Akt1) or PKBgamma (Akt3).
Storage	-20ºC
Storage Buffer	TBS, 50% glycerol, 0.01% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, WB, ICC/IF
Description	WB ; ICC ; IF ; ELISA
Dilution	WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64. 3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906755-1	SPC-148F	Unconjugated	400 ul	$289	Add to Cart
BHA11906755-2	SPC-148F-A390	ATTO 390	400 ul	$343	Add to Cart
BHA11906755-3	SPC-148F-A488	ATTO 488	400 ul	$342	Add to Cart
BHA11906755-4	SPC-148F-A594	ATTO 594	400 ul	$342	Add to Cart
BHA11906755-5	SPC-148F-BI	Biotin	400 ul	$338	Add to Cart
BHA11906755-6	SPC-148F-FITC	FITC	400 ul	$333	Add to Cart

Akt2 (PKB beta) Antibody - SPC-148