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Home / Antibodies

AKT1 Antibody (V3799SAF)

SKU : BHA17125623

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NSJ Bioreagents

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Mfr.No.	V3799SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-beta or RacPK-beta) and Akt 3 (also designated PKB-gamma or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human
Note	Titering of the AKT1 antibody may be required for optimal performance.

Uniprot	P31749

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125623	V3799SAF-100UG	100 ug	$499		Add to Cart

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