Designed to perform easy wash steps using a standard 96-well filter plate and the ANTIGENIX Super-X Plex™ bead assay for flow cytometric analysis.
Intended Use
Un-pack the components and attached the supplied tubing to a 1 or 2 litre vacuum flask (not supplied). The ON/OFF valve should be positioned BETWEEN the flask and the Washer. The outlet of the Vacuum Flask is connected to customer's vacuum system. An In-Line HEPA filter to protect the vacuum system is also recommended. 2. Filter plate filled with 100 uL Wash buffer is placed on top of washer. Turn-ON the vacuum supply. 3. Press evenly on all corners of the Filter Plate to provide tight seal. 4. Turn Vacuum OFF as soon as buffer solution filters through wells - Remove the plate.
Components
Filter Plate Washer and tubing with ON/OFF valve assembly.
Important Notes
Adjust vacuum system pressure - so that buffer solution filters through plate in approx. 3-5 seconds. rnVacuum pressure set too low can result in loss of washing efficiency, and too high can result in bead loss.
Notes
Connecting to an overflow flask and an in-line High Efficiency Particulate Air(HEPA) filter to protect the vacuum system is recommended.