Specialized Production of High-Quality AAV Libraries
AAVnerGene excels in producing high-quality, diverse AAV libraries, with a particular focus on the challenging AAV Capsid Library(II). This type of library production is technically demanding due to the propensity of AAV library variants to experience cross-packaging and capsid mosaic formation, particularly at high plasmid levels. A key strategy to mitigate these issues involves producing the AAV capsid library at low copy numbers, thereby reducing the likelihood of cross-packaging and capsid mosaic formation.
Overcoming Production Challenges
However, lowering the library plasmid copy number during transfection presents its own set of challenges, notably the efficient and reproducible generation of a large number of AAV vectors. To address this, AAVnerGene leverages the mini-pHelper-Rep plasmid in conjunction with high-efficiency suspension HEK 293T cell lines. This approach has made the production of AAV Capsid Library(II) more accessible and affordable, benefiting both researchers and biotechnology companies.