rAAV-EF1a-DIO-mCherry-WPRE-hGH polyA

Mfr.No.	PT-0013/BC-0016
Description	Cre dependent expression of the mCherry driven by the ubiquitous EF1a promoter
Function	Control/Fluorescent Proteins
Promoter	EF1a
Insert Gene	/
Fluorophore/Tag	mCherry
Activity	Cre-dependent
Serotype	Available serotypes: AAV2/1; AAV2/5; AAV2/8; AAV2/9; AAV2/DJ; AAV2/Retro; AAV2/PHP.eB; AAV2/2; AAV2/6; AAV2/rh10; AAV2/PHP.S; AAV2/anc80L65; AAV2/B10; AAV2/olig001; AAV2/6.2FF; AAV2/BI30; AAV2/7m8; AAV2/BR1; AAV2/6m; AAV2/MG1.2; AAV1/2; AAV2/MaCPNS1; AAV2/MaCPNS2; AAV2/DJ8; AAV2/PHP.B
Titer	>=2.00E+12 vg/mL; For higher titer (>=1.00E+13 vg/mL), please inquire.
Storage	Should be stored at -80°C

Reference Paper	Orexin activated emergence from isoflurane anaesthesia involves excitation of ventral tegmental area dopaminergic neurones in rats J Li, H Li, D Wang, Y Guo, X Zhang, M Ran… - British journal of …, 2019 - Elsevier … Adeno-associated viruses expressing channelrhodopsin 2 (rAAV-Ef1α-DIO-hChR2-mCherry, ChR2), eNpHR3.0 (rAAV-Ef1α-DIO-NpHR-mCherry, NpHR), or control virus (rAAV-Ef1α-DIO- mCherry, mCherry) (Brain-VTA, Wuhan, China) were microinjected into the PeFLH (anterior … Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice S Zhao, R Li, H Li, S Wang, X Zhang, D Wang… - Neuroscience …, 2021 - Springer … Viruses (Brain-VTA, Wuhan, China) were then microinjected into the LHA. For pharmacogenetic ablation tests, 200 nL rAAV-Ef1α-DIO-taCasp3-TEVP (Caspase 3) or rAAV- Ef1α-DIO-mCherry (Control) were bilaterally microinjected into the LHA (AP –1.80 mm, ML ±0.9 mm, DV BNSTAVGABA-PVNCRF Circuit Regulates Visceral Hypersensitivity Induced by Maternal Separation in Vgat-Cre Mice ST Huang, ZJ Song, Y Liu, WC Luo, Q Yin… - Frontiers in …, 2021 - frontiersin.org … Adeno-Associated Virus (AAV) Tools. PVN-microinjection: rAAV-CRF-EYFP-WPRE- pA (2.04 ×10 ⁠12 vg/ml, Customized, BrainVTA, China) ; rAAV-Ef1α-DIO-mCherry- WPRE-pA (retro) (2.06 ×10⁠ 12 vg/ml, BrainVTA, China) … Optogenetic/chemogenetic activation of GABAergic neurons in the ventral tegmental area facilitates general anesthesia via projections to the lateral … L Yin, L Li, J Deng, D Wang, YX Guo… - Frontiers in neural …, 2019 - frontiersin.org … For chemogenetic experiments, AAV-Ef1α-DIO-hM3Dq-mCherry, AAV-Ef1α DIO-hM4Di- mCherry, or rAAV-Ef1α-DIO-mCherry (BrainVTA, China) were bilaterally injected into the VTA as described above. After microinjection, the incision was sutured … Disinhibition of PVN-projecting GABAergic neurons in AV region in BNST participates in visceral hypersensitivity in rats Y Song, QX Meng, K Wu, R Hua, ZJ Song… - …, 2020 - Elsevier … rAAV-Vgat-EYFP-WPRE-pA (2.04 × 10 12 vg/mL, PT-0190, BrainVTA, China); rAAV-Ef1α-DIO- mCherry-WPRE-pA (2.06 × 10 12 vg/mL, PT-0013, BrainVTA, China); rAAV-Ef1α-DIO-hM3D( Gq)-mCherry-WPRE-pA (2.41 × 10 12 vg/mL, PT-0042, BrainVTA, China); rAAV-Ef1α-DIO … Dopaminergic Projections From the Ventral Tegmental Area to the Nucleus Accumbens Modulate Sevoflurane Anesthesia in Mice H Gui, C Liu, H He, J Zhang, H Chen… - Frontiers in Cellular …, 2021 - ncbi.nlm.nih.gov … All viruses purchased from Brain-VTA (Wuhan, China … EGFP, PT-0043), optogenetic (rAAV2/9-EF1α-DIO-ChR2-mCherry, PT-0002; rAAV2/9-EF1α-DIO-NpHR-mCherry, PT-0007), Retro-cre (rAAV2/Retro-hSyn-cre-mCherry, PT-0407), mCherry (rAAV-Ef1α-DIO-mCherry-WPRE … Progress in Neuropsychopharmacology & Biological Psychiatry Y Wang, C Zuo, Q Xu, L Hao, Y Zhang - researchgate.net … voles received an injection of the rAAV- EF1α-DIO-hM3Dq-mCherry (anterogradely travelling adenoassociated AAV2/9, under a double-floxed inverted open-reading frame construct (DIO), encoding hM3D (Gq)-mCherry), or rAAV-EF1α-DIO-mCherry (Brain VTA, Wuhan, China) …

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Combination SKU	Supplier No.	Serotype	Size	Your price
BHV12400236-1	PT-0013/BC-0016	AAV2/1	100ul	$531	Add to Cart
BHV12400236-2	PT-0013/BC-0016	AAV2/2	100ul	$531	Add to Cart
BHV12400236-3	PT-0013/BC-0016	AAV2/5	100ul	$531	Add to Cart
BHV12400236-4	PT-0013/BC-0016	AAV2/6	100ul	$531	Add to Cart
BHV12400236-5	PT-0013/BC-0016	AAV2/6m	100ul	$531	Add to Cart
BHV12400236-6	PT-0013/BC-0016	AAV2/6.2FF	100ul	$531	Add to Cart
BHV12400236-7	PT-0013/BC-0016	AAV2/7m8	100ul	$531	Add to Cart
BHV12400236-8	PT-0013/BC-0016	AAV2/8	100ul	$531	Add to Cart
BHV12400236-9	PT-0013/BC-0016	AAV2/9	100ul	$531	Add to Cart
BHV12400236-10	PT-0013/BC-0016	AAV2/DJ	100ul	$531	Add to Cart
BHV12400236-11	PT-0013/BC-0016	AAV2/DJ8	100ul	$531	Add to Cart
BHV12400236-12	PT-0013/BC-0016	AAV2/Retro	100ul	$531	Add to Cart
BHV12400236-13	PT-0013/BC-0016	AAV2/PHP.eB	100ul	$531	Add to Cart
BHV12400236-14	PT-0013/BC-0016	AAV2/PHP.B	100ul	$531	Add to Cart
BHV12400236-15	PT-0013/BC-0016	AAV2/rh10	100ul	$531	Add to Cart
BHV12400236-16	PT-0013/BC-0016	AAV2/PHP.S	100ul	$531	Add to Cart
BHV12400236-17	PT-0013/BC-0016	AAV2/anc80	100ul	$531	Add to Cart
BHV12400236-18	PT-0013/BC-0016	AAV2/B10	100ul	$531	Add to Cart
BHV12400236-19	PT-0013/BC-0016	AAV2/olig001	100ul	$531	Add to Cart
BHV12400236-20	PT-0013/BC-0016	AAV2/BI30	100ul	$531	Add to Cart
BHV12400236-21	PT-0013/BC-0016	AAV2/BR1	100ul	$531	Add to Cart
BHV12400236-22	PT-0013/BC-0016	AAV2/MG1.2	100ul	$531	Add to Cart
BHV12400236-23	PT-0013/BC-0016	AAV1/2	100ul	$531	Add to Cart
BHV12400236-24	PT-0013/BC-0016	AAV2/MaCPNS1	100ul	$531	Add to Cart
BHV12400236-25	PT-0013/BC-0016	AAV2/MaCPNS2	100ul	$531	Add to Cart

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rAAV-EF1a-DIO-mCherry-WPRE-hGH polyA

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Optogenetic/chemogenetic activation of GABAergic neurons in the ventral tegmental area facilitates general anesthesia via projections to the lateral …

Disinhibition of PVN-projecting GABAergic neurons in AV region in BNST participates in visceral hypersensitivity in rats

Dopaminergic Projections From the Ventral Tegmental Area to the Nucleus Accumbens Modulate Sevoflurane Anesthesia in Mice

Progress in Neuropsychopharmacology & Biological Psychiatry

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