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Integrated AAV Offerings: From Cutting-Edge Products to Advanced Custom Services
Overcoming AAV Technology Challenges with Expertly Tailored Vector Design and Production
AAV Vector Design Services
Adeno-associated virus (AAV) vectors are widely used in gene delivery and gene therapy due to their efficiency and safety. The first step in successful AAV vector-based research is designing a shuttle plasmid that can be effectively packaged into an AAV capsid. This process involves optimizing transgene expression strategies, selecting appropriate tags, managing AAV genome sizes, and choosing the right AAV plasmid backbones to ensure high transgene expression, efficient packaging, and targeted delivery of the AAV vector.
AAV Vector Design
We offer One-Stop Services for the design, construction, and cloning of customized AAV vectors. Our team of molecular and AAV experts provides comprehensive support throughout the AAV vector design process to meet your specific needs.
Services We Offer
| AAV Plasmid Backbones Selection | Choose from our range of plasmid backbones, including pAAVone, pAAVdual, and pAAVtri. We provide options for single-stranded AAV (ssAAV), self-complementary AAV (scAAV), and different packaging capacities. |
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| Promoters and Regulatory Elements Selection | Guidance on selecting suitable promoters (e.g., CMV, CAG, uBC, EF1a) and other regulatory elements for precise gene expression. |
| Expression and Labeling Strategies | Custom expression strategies and labeling methods to achieve targeted gene delivery. |
| AAV Serotype Selection | Assistance in selecting the optimal AAV serotype for your research application. |
| Vector Optimization | Optimization of vector design for efficient packaging and high transgene expression |
| AAV Production Systems | We offer several production systems, including: AAVone: Single-plasmid system for efficient AAV production. AAVdual: Two-plasmid system for flexible vector production. AAVtri: Triple-plasmid system for more complex applications. |
| Troubleshooting and Experimental Design | Comprehensive troubleshooting and support to ensure the success of your AAV vector experiments. |
| DNA Preparation and Cloning Services | Preparation of DNA Fragments: Digestion and Amplification: We provide services for the digestion of plasmids and amplification of requested sequences. Synthesis of DNA Fragments: Low-cost DNA synthesis at $0.2 per base pair. |
| Molecular Cloning: Cloning using standard techniques (e.g., Gibson Assembly, Gateway Cloning) with options for assembling multiple fragments simultaneously. |
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| Quality Control: Restriction Enzyme Digestion: To confirm the accuracy of clones. SmaI/AdhI Digestion: Verification of intact Inverted Terminal Repeats (ITRs). Sanger Sequencing: To verify inserts and, upon request, perform whole plasmid sequencing. |
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| Delivery and Documentation: Delivery of Plasmid DNA: We provide mini-scale preparations of plasmid DNA; larger scales are available upon request. Detailed Reporting: A comprehensive report summarizing experimental strategies, raw data, and final results. |
Pricing and Turnaround
| Service | Description | Price | Turnaround |
|---|---|---|---|
| Gene Synthesis | Regular gene/DNA fragment synthesis | $0.20/bp | 1-2 weeks |
| Difficult Gene Synthesis | GC-rich, high secondary structure DNAs | $0.35/bp | 2-3 weeks |
| Plasmid Clone | Cloning any fragments into AAV backbone | $300 | 1-2 weeks |
| Mini Prep | 10 µg plasmid, endotoxin-free | $100 | 3 days |
| Midi Prep | 100 µg of endotoxin-free plasmid | $150 | 3-5 days |
| Maxi Prep | 400 µg of endotoxin-free plasmid | $200 | 3-5 days |
| Mega Prep | 2,000 µg of endotoxin-free plasmid | $500 | 3-5 days |
| Giga Prep | 10,000 µg of endotoxin-free plasmid | $1,000 | 5-7 days |
Key Elements in AAV Plasmid Design
- Inverted Terminal Repeats (ITRs):
- Essential for AAV replication and packaging; ensure functional ITRs before AAV packaging.
- Promoters:
- Select appropriate promoters for targeted gene expression.
- Introns:
- Enhance transcript stability and translation efficiency.
- Gene of Interest (Transgene):
- Ensure compatibility with AAV vector packaging capacity.
- Polyadenylation (PolyA) Signal:
- Crucial for mRNA maturation and stability.
- Enhancers and Regulatory Elements:
- Boost and fine-tune gene expression, such as using WPRE for enhanced promoter activity.
- Selectable Marker and Origin of Replication:
- Ensure plasmid propagation and selection during bacterial culture.
Contact US for Custom Services
Interested in AAV Vector Design Services tailored to your specific research needs? Contact our technical team today to discuss your project requirements.
AAV Plasmid Backbones
Our AAV plasmids are available in various configurations to suit different research needs:| Backbone Type | Backbone Size | Selection Marker | Ori | Ad Genes | Rep/Cap | Genome Type | Production System |
|---|---|---|---|---|---|---|---|
| pAAVtri | 2.6 kb | Kana | pUC/F1 | No | No | ssAAV | AAVtri |
| pAAVtri-sc | 2.6 kb | Kana | pUC/F1 | No | No | scAAV | AAVtri |
| pAAVdual | 8.4 kb | Kana | pUC/F1 | Yes | No | ssAAV | AAVdual |
| pAAVdual-sc | 8.4 kb | Kana | pUC/F1 | Yes | No | scAAV | AAVdual |
| pAAVone | 13 kb | Kana | pUC/F1 | Yes | Yes | ssAAV | AAVone |
| pAAVone-sc | 13 kb | Kana | pUC/F1 | Yes | Yes | scAAV | AAVone |
AAV Services
Custom AAV Vector Design and Cloning
Expert consultation and design of custom AAV vectors tailored to your research needs.
AAV Production Services
Scalable production services from small pilot batches to large-scale bioreactor production.
AAV Purification Services
High-quality purification using state-of-the-art techniques like affinity chromatography.
AAV Quality Control and Characterization
Comprehensive quality control assays including qPCR, SDS-PAGE, and next-generation sequencing.
Preclinical Development Services
In vitro and in vivo studies to evaluate transduction efficiency, tissue tropism, and safety.
Ready-to-Use AAV Vectors
Off-the-shelf AAV vectors for standard applications and proof-of-concept studies.
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