Adeno-Associated Virus (AAV) Custom Production Service

Key informations
● The packaging capacity is~4.7 kb from ITR to ITR for single stranded AAV and ~2.2 kb from ITR to ITR for self-complementary AAV.
● Viral particles are purified by ultracentrifugation on an iodixanol step gradient.
● Quality control assays include qPCR to quantify viral titers and silver stain to determine viral titer and purity after purification.
● Serotypes include 1, 2, 5, 6, 8, 9, rh10, DJ, DJ8, PHP.B, PHP.eB, PHP.S, retro, anc80. Novel peptide modified serotypes can also be produced on a case-by-case basis. For information on Serotype and Tissue Specificity, please visit introduction to AAV Services.
● Here are some examples of different types of custom-made AAV vectors that have been produced successfully:
-CRISPR/ Cas9
-Optogenetics
-DREADDS
-Calcium sensors
-Cre / FlpO
-Tracing virus helper
-Overexpression
-RNAi
AAV specific infection in some organs and tissues.
    
Large Scale Custom-Made AAVs Production
BrainVTA has established a serum-free cell suspension culture system, through which we offer large scale production with serum free, antibiotic free, highly purified and highly potent products.

For customers 
Find out our custom-Made AAV service following the guide below:
● Submit your needs to us by clicking the Send Request button or email us at [email protected] and our experienced scientists will design your vector for free.
● Or, for any customers who decides to take use of viruses as nucleotide sequence delivery tools, our scientists will work with you step-by-step from design of study to viral manufacturing to facilitate your research.

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