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AAV Plasmid Vectors
AAV plasmids are essential components in the production of Adeno-Associated Virus (AAV) vectors. Traditional transient transfection method involves co-transfecting HEK293 cells with three plasmids containing the necessary cis and trans components for AAV replication and propagation: pAAV-GOI, pRep-Cap, and pHelper. >> Click Here to Learn More About Each AAV Plasmid and Their Roles in AAV Production
In collaboration with AAVnerGene Inc., we offer AAV plasmids designed for different production systems, including AAVone, AAVdual, and AAVtri. Each system is tailored to meet specific vector design and production needs, providing flexibility and scalability in AAV production. These systems are optimized to enhance the efficiency and yield of AAV vector production, accommodating various research and therapeutic applications.
Figure 1. An illustrative diagram outlining the AAV plasmids required in different production systems - Traditional triple-plasmid system, AAVnerGene's proprietary AAVtri system, their proprietary AAVdual system, and proprietary AAVone system.
AAV Plasmids by Production System
| Production System | Plasmid Backbone | Examples | Ad Genes | Rep/Cap | Genome |
| AAVone System | pAAVone | pAAVone-AAV9-CMV-EGFP | Yes | Yes | ssAAV |
| pAAVone-sc | pAAVone-sc-AAV9-CMV-EGFP | Yes | Yes | scAAV | |
| AAVdual System | pAAVdual | pAAVdual-CMV-EGFP | Yes | No | ssAAV |
| pAAVdual-sc | pAAVdual-sc-CMV-EGFP | Yes | No | scAAV | |
| AAVtri System | pAAVtri | pAAVtri-CMV-EGFP | No | No | ssAAV |
| pAAVtri-sc | pAAVtri-sc-CMV-EGFP | No | No | scAAV | |
| Ad helper | mini-pHelper | Yes | No | No |
Custom AAV Services
AAV Vector Design & Cloning
Expert consultation, design, gene synthesis, and cloning of custom AAV vectors tailored to your research needs.
Learn moreAAV Packaging Service
Reliable, affordable, and fully customizable AAV packaging service with diverse serotype options and high titer.
Learn moreAAV QC & Characterization
Comprehensive quality control assays including qPCR, SDS-PAGE, mass photometry, and next-generation sequencing.
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