JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....

AAV Plasmid Vectors

AAV Plasmid Vectors

AAV Plasmid Vectors

AAV plasmids are essential components in the production of Adeno-Associated Virus (AAV) vectors. Traditional transient transfection method involves co-transfecting HEK293 cells with three plasmids containing the necessary cis and trans components for AAV replication and propagation: pAAV-GOI, pRep-Cap, and pHelper. >> Click Here to Learn More About Each AAV Plasmid and Their Roles in AAV Production

In collaboration with AAVnerGene Inc., we offer AAV plasmids designed for different production systems, including AAVone, AAVdual, and AAVtri. Each system is tailored to meet specific vector design and production needs, providing flexibility and scalability in AAV production. These systems are optimized to enhance the efficiency and yield of AAV vector production, accommodating various research and therapeutic applications.





Figure 1. An illustrative diagram outlining the AAV plasmids required in different production systems - Traditional triple-plasmid system, AAVnerGene's proprietary AAVtri system, their proprietary AAVdual system, and proprietary AAVone system.

AAV Plasmids by Production System

 
Production System Plasmid Backbone Examples Ad Genes Rep/Cap Genome
AAVone System  pAAVone pAAVone-AAV9-CMV-EGFP  Yes Yes ssAAV
pAAVone-sc pAAVone-sc-AAV9-CMV-EGFP  Yes Yes scAAV
AAVdual System  pAAVdual pAAVdual-CMV-EGFP  Yes No ssAAV
pAAVdual-sc pAAVdual-sc-CMV-EGFP  Yes No scAAV
AAVtri System  pAAVtri pAAVtri-CMV-EGFP  No No ssAAV
pAAVtri-sc pAAVtri-sc-CMV-EGFP No No scAAV
Ad helper mini-pHelper Yes No No

Custom AAV Services

AAV Vector Design & Cloning

Expert consultation, design, gene synthesis, and cloning of custom AAV vectors tailored to your research needs.

Learn more

AAV Packaging Service

Reliable, affordable, and fully customizable AAV packaging service with diverse serotype options and high titer.

Learn more

AAV QC & Characterization

Comprehensive quality control assays including qPCR, SDS-PAGE, mass photometry, and next-generation sequencing.

Learn more

Other Viruses at Biohippo

Rabies Virus (RV)

Neural Circuit Tracing

Biohippo offers a variety of viral vectors, including RV, HSV, PRV, and VSV, to replace conventional tracers with the highly efficient, more specific, and less invasive viral method.

Learn More
Rabies Virus (RV)

Lentivirus

Biohippo offers TF reporter lentivirus, immunotherapy lentivirus, validated shRNA, cDNA, and miRNA lentiviruses to meet diverse research needs. We also offer customized service at affodable price.

Learn More