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Integrated AAV Offerings: From Cutting-Edge Products to Advanced Custom Services
Overcoming AAV Technology Challenges with Expertly Tailored Vector Design and Production
AAV Packaging Services Form
To provide you with an accurate price quote and ensure a smooth service process, we kindly request you to provide the following information:
Notes
Ship your plasmid to the following address:
Attn: Biohippo Inc.
Phone: 866-986-9598
Address: 900 Clopper Rd, Suite 140, Gaithersburg, MD 20878
Plasmid(s) for packaging: You may ship your endotoxin-free plasmids in an eppendorf tube to our address. We require at least 0.3mg/1E+13GC virus. If you are unable to provide plasmids in the requested amount, we offer maxiprep service at an additional charge of $200, and we require at least 10μg raw material plasmid from the customer to start with.
Productivity: The productivity is estimated based on the average yield of common transgene vectors of the same serotype using rep-cap plasmid. If the productivity is significantly lower than average due to customer's plasmid vector (e.g. virus genome size exceeds 4.7kb), a scale-up production with additional chrage may be required to deliver the requested amount.
Packaging Method: HEK293T cells will be used for AAV packaging by transient transfection of plasmid.
Purification Method: AAV vector will be purified by two rounds of CsCl, or iodixanol gradient ultracentrifugation, or by affinity chromatography followed by one round of CsCl ultracentrifugation to get rid of host cell contaminate and enrich AAV capsids with full genome.
Titer Measurement: The titer is measured by SYBRGreen qPCR using primers targeting gene-specific sequences. By default, we will measure ITR titer, but please let us know if you would like use other common targets: CMV, EF1a, GFAP, Syn, eGFP, mCherry, WPRE, WPRE3, bGH.
Formulation Buffer: Our default formulation bufer is 1x DPBS plus additional 150mM NaCl and 0.001% Pluronic F-68. Please let us know if you have your own buffer system.
