Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-357 with an N-terminal His-tag, MW=42.8 kDa, expressed in an E. coli cell expression system.
Specific activity: 10.5 pmol/min/ug
Immunogen Region
2-357
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and A20. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, and emission = 460 nm.
Avoid freeze/thaw cycles.
GenBank
NM_006290
Mw(kda)
42.8 kDa
Synonyms
A20, tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C
Uniprot
P21580
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7. 2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9. 3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41