Human PIgMent Epithelium-derived Factor

Mfr.No.

E1634Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Pigment Epithelium-Derived Factor (also known as PEDF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cell proliferation-inducing gene 35 protein;EPC-1;PEDF;Pigment epithelium-derived factor;Serpin F1;SERPINF 1;SERPINF1

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

46312

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.017ng/ml

Background

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Gene ID	5176
UniProt ID	P36955
Antigen	SERPINF1

Research Area

Cancer

Protocol

These standard curves of E1634Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.117	2.149	2.105
	2.181
6ng/ml	1.238	1.319	1.274
	1.4
3ng/ml	0.656	0.692	0.647
	0.728
1.5ng/ml	0.311	0.372	0.328
	0.433
0.75ng/ml	0.119	0.141	0.096
	0.162
0ng/ml	0.045	0.044	0
	0.043

Precision

We measured random samples of E1634Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.4	0.21	4.9
Intra-Assay	2	18	7.4	0.27	3.6
Intra-Assay	3	18	3.1	0.18	5.9

Storage	2-8°C

Reference	Core-shell fibrous scaffold as a vehicle for sustained release of retinal pigmented epithelium?derived factor (PEDF) for photoreceptor differentiation of conjunctiva mesenchymal stem cells Journal: J Biomed Mater Res A. 2017 Dec;105(12):3514-3519.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103219	E1634Hu	48T	$320		Add to Cart
BHE12103219	E1634Hu	96T	$458		Add to Cart

Human PIgMent Epithelium-derived Factor - PEDF ELISA Kit

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