Human Lymphotoxin-beta - LTB ELISA Kit

Mfr.No.

E0171Hu

Description

This sandwich kit is for the accurate quantitative detection of human Lymphotoxin-beta (also known as LTB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

LT-beta;Lymphotoxin-beta;TNF C;TNFC;TNF-C;Tumor necrosis factor C;Tumor necrosis factor ligand superfamily member 3

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

25390

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.98ng/L

Background

Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional.

Gene ID	4050
UniProt ID	Q06643
Antigen	TNFC

Research Area

Others

Protocol

These standard curves of E0171Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.361	2.236	2.194
	2.112
1200ng/L	1.926	1.814	1.772
	1.702
600ng/L	1.209	1.149	1.107
	1.09
300ng/L	0.65	0.628	0.586
	0.607
150ng/L	0.376	0.362	0.319
	0.348
0ng/L	0.044	0.043	0
	0.041

Precision

We measured random samples of E0171Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1015.7	45.77	4.5
Intra-Assay	2	18	556.3	30.07	5.4
Intra-Assay	3	18	251.5	12.34	4.9

Storage	2-8°C

Reference	Effects of Myrtus communis extract treatment in bile duct ligated rats Journal: J Surg Res. 2016 Oct;205(2):359-367.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101906	E0171Hu	96T	$458		Add to Cart
BHE12101906	E0171Hu	48T	$320		Add to Cart

Human Lymphotoxin-beta - LTB ELISA Kit

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