Human Interleukin 18 - IL-18 ELISA Kit

Mfr.No.

E0147Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 18 (also known as IL-18) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Iboctadekin;IFN-gamma-inducing factor;IL 18;IL-1 gamma;IL18;IL-18;Interferon gamma-inducing factor;Interleukin-1 gamma;Interleukin-18;

Reactivity

Human

Detection Range

0.5-100ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22326

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.2ng/L

Background

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed: 14528293, PubMed: 25500532). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable) (PubMed: 10653850).

Gene ID	3606
UniProt ID	Q14116
Antigen	IL18

Research Area

Immunology

Protocol

These standard curves of E0147Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/L	2.016	2.103	2.064
	2.19
32ng/L	1.301	1.358	1.319
	1.415
16ng/L	0.723	0.651	0.612
	0.579
8ng/L	0.421	0.386	0.347
	0.351
4ng/L	0.147	0.157	0.118
	0.167
0ng/L	0.031	0.039	0
	0.046

Precision

We measured random samples of E0147Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	41.4	1.67	4
Intra-Assay	2	18	24.4	1.06	4.3
Intra-Assay	3	18	8.5	0.38	4.5

Storage	2-8°C

Reference	Efficacy of multiple Biomarkers: NGAL, KIM1, Cystatin C and IL18 in predicting pregnancy related acute kidney injury Journal: Pak J Med Sci. 2023 Jan-Feb;39(1):34-40. Caspase-1 and interleukin-18 in children with post infectious bronchiolitis obliterans: a case-control study Journal: Eur J Pediatr. 2022 Aug;181(8):3093-3101. Apolipoprotein A2? 265 T> C polymorphism interacts with dietary fatty acids intake to modulate inflammation in type 2 diabetes mellitus patients Journal: Nutrition. 2017 May;37:86-91. Predictive Values of Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin for Delayed Graft Function Diagnosis in Kidney Transplantation Journal: Iran J Pathol. 2016 Fall;11(4):391-398. Effect of Hypertonic Saline 5% on Early Graft Function and Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin in Deceased-Donor Kidney Transplantation Journal: Iran J Pharm Res. 2017 Fall;16(4):1583-1592. The effect of magnesium sulfate on gene expression and serum level of inflammatory cytokines in coronary artery disease patients Journal: Inflammopharmacology. 2023 Oct;31(5):2421-2430. Interleukin-18 serum level before and after intralesional immunotherapy with tuberculin purified protein derivative in patients with cutaneous and genital warts Journal: J Cosmet Dermatol. 2022 Dec;21(12):7035-7042. Caspase-1 and interleukin-18 in children with post infectious bronchiolitis obliterans: a casecontrol study Journal: Eur J Pediatr. 2022 Aug;181(8):3093-3101.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101882	E0147Hu	96T	$458		Add to Cart
BHE12101882	E0147Hu	48T	$320		Add to Cart

Human Interleukin 18 - IL-18 ELISA Kit

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