Human Interleukin 1beta - IL-1B ELISA Kit

Mfr.No.

E0143Hu

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 1Beta (also known as IL-1Beta) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Catabolin;IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Reactivity

Human

Detection Range

20-6000pg/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

30748

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.07pg/mL

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed: 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed: 12794819).

Gene ID	3553
UniProt ID	P01584
Antigen	IL1B

Research Area

Immunology; Microbiology; Cardiovascular; Metabolism

Protocol

These standard curves of E0143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000pg/mL	2.364	2.272	2.23
	2.18
2000pg/mL	1.378	1.317	1.275
	1.256
1000pg/mL	0.807	0.724	0.682
	0.642
500pg/mL	0.443	0.463	0.421
	0.483
250pg/mL	0.195	0.208	0.166
	0.222
0pg/mL	0.027	0.042	0
	0.058

Precision

We measured random samples of E0143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1899.9	61.65	3.2
Intra-Assay	2	18	821.3	40.18	4.9
Intra-Assay	3	18	1765.1	88.88	5

Storage	2-8°C

Reference	Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors Journal: Nutr Metab Cardiovasc Dis. 2020 Apr 12;30(4):674-682 The Potential Predictive Role of Tumour Necrosis Factor-?, Interleukin-1?, and Monocyte Chemoattractant Protein-1 for COVID-19 Patients Survival Journal: Infect Drug Resist. 2022 Mar 4;15:821-829 Evaluation of periodontal status and cytokine response in children with familial Mediterranean fever or systemic juvenile idiopathic arthritis Journal: Clin Oral Investig. 2022 Oct 5 The Relationship between Serum Concentrations of Pro- and Anti-Inflammatory Cytokines and Nutritional Status in Patients with Traumatic Head Injury in the Intensive Care Unit Journal: Medicina (Kaunas). 2019 Aug 15;55(8):486 Correlation between serum pro inflammatory cytokines and clinical scores of knee osteoarthritic patients using resveratrol as a supplementary therapy with meloxicam Journal: Indian J Pharmacol. 2021 Jul-Aug;53(4):270-277. The Potential Predictive Role of Tumour Necrosis Factor-?, Interleukin-1?, and Monocyte Chemoattractant Protein-1 for COVID-19 Patients Survival Journal: Infect Drug Resist. 2022 Mar 4;15:821-829. Evaluation of periodontal status and cytokine response in children with familial Mediterranean fever or systemic juvenile idiopathic arthritis Journal: Clin Oral Investig. 2023 Mar;27(3):1159-1166. The Relationship between Serum Concentrations of Pro- and Anti-Inflammatory Cytokines and Nutritional Status in Patients with Traumatic Head Injury in the Intensive Care Unit Journal: Medicina (Kaunas). 2019 Aug 15;55(8):486. Effect of high amylose resistant starch (HAM?RS2) supplementation on biomarkers of inflammation and oxidative stress in hemodialysis patients: a randomized clinical trial Journal: Hemodial Int. 2018 Oct;22(4):492-500. Correlation Between IL-8, C-Reactive Proteins (CRP) and Neutrophil to Lymphocyte Ratio (NLR) as Predictor of Mortality in COVID-19 Patients with Diabetes Mellitus Comorbidity Journal: Int J Gen Med. 2023 Jun 8;16:2349-2354. Effects of autologous leukocyte-platelet rich fibrin (L-PRF) on the rate of maxillary canine retraction and various biomarkers in gingival crevicular fluid (GCF): A split mouth randomized controlled trial Journal: Int Orthod. 2022 Dec;20(4):100681.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101878	E0143Hu	96T	$458		Add to Cart
BHE12101878	E0143Hu	48T	$320		Add to Cart

Human Interleukin 1beta - IL-1B ELISA Kit

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