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Home / Primary Antibodies

Phospho-YB1 (Tyr162) Antibody

SKU : BHA12912195

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF7333
Alternative Names	BP 8 ; CBF-A ; CCAAT binding transcription factor I subunit A ; CCAAT-binding transcription factor I subunit A ; CSDA2 ; CSDB ; DBPB ; DNA binding protein B ; DNA-binding protein B ; EFI-A ; Enhancer factor I subunit A ; MDR NF1 ; MGC104858 ; MGC110976 ; MGC117250 ; NSEP 1 ; NSEP1 ; Nuclease sensitive element binding protein 1 ; Nuclease-sensitive element-binding protein 1 ; p50 ; Q15905 ; Y-box binding protein 1 ; Y-box transcription factor ; Y-box-binding protein 1 ; YB 1 ; YB-1 ; YBOX1_HUMAN ; YBX 1 ; ybx1 ;
Antigen	Phospho-YB1 (Tyr162)
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human YB1 around the phosphorylation site of Tyr162.
Molecular Weight	Observed Mol.Wt.: 50kDa. Predicted Mol.Wt.: 36kDa.
Reactivity	Human, Mouse, Rat
Specificity	Phospho-YB1 (Tyr162) Antibody detects endogenous levels of YB1 only when phosphorylated at Tyr162.
Subcellular Location	Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.

Uniprot

P67809

Gene Id

4904

Sequence

10 20 30 40 50
MSSEAETQQP PAAPPAAPAL SAADTKPGTT GSGAGSGGPG GLTSAAPAGG
60 70 80 90 100
DKKVIATKVL GTVKWFNVRN GYGFINRNDT KEDVFVHQTA IKKNNPRKYL
110 120 130 140 150
RSVGDGETVE FDVVEGEKGA EAANVTGPGG VPVQGSKYAA DRNHYRRYPR
160 170 180 190 200
RRGPPRNYQQ NYQNSESGEK NEGSESAPEG QAQQRRPYRR RRFPPYYMRR
210 220 230 240 250
PYGRRPQYSN PPVQGEVMEG ADNQGAGEQG RPVRQNMYRG YRPRFRRGPP
260 270 280 290 300
RQRQPREDGN EEDKENQGDE TQGQQPPQRR YRRNFNYRRR RPENPKPQDG
310 320
KETKAADPPA ENSSAPEAEQ GGAE

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, IHC, WB
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12912195	AF7333	100ul	$350		Add to Cart
BHA12912195	AF7333	200ul	$450		Add to Cart

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