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Anti-MTOR Antibody BHA11300952
Alternative: Serine/threonine-protein kinase mTOR|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP12-rapamycin complex-associated protein|Mammalian target of rapamycin|mTOR|Mechanistic target of rapamycin|Rapamycin and FKBP12 target 1|Rapamycin target protein 1|MTOR|FRAP|FRAP1|FRAP2|RAFT1|RAPT1
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: primary antibodies|polyclonal antibodies
Chromosome Location: 1p36.22
Immunogen: Synthetic peptide, corresponding to amino acids 2430-2480 of Human mTOR.
Description: mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
TARGETWeight: 289 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: mTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein.
Description: Western blot (WB) analysis of mTOR (S2442) at 1:500 dillutionLane1:NIH-3T3 whole cell lysate(40μg)Lane2:HEK293T whole cell lysate(40μg)Lane3:H9C2 whole cell lysate(40μg)Lane4:Hela whole cell lysate(40μg)Lane5:MCF-7 whole cell lysate(40μg)Lane6:HepG2 whole cell lysate(40μg)
Description: Immunohistochemistry (IHC) analyzes of mTOR (S2442) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Description: Immunoprecipitation of SGC7901 cell lysates using mTOR (S2442) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using mTOR (S2442) pAb #BS1844.“↑”(supernatant); “↓”(deposition).
Description: Western blot (WB) analysis of mTOR (S2442) pAb at 1:500 dilutionLane1:H9C2 whole cell lysate(40ug)Lane2:AML-12 whole cell lysate(40ug)Lane3:HepG2 whole cell lysate(40ug)Lane4:L02 whole cell lysate(40ug)
Description: Western blot analysis of extracts from HEK293T cells transfected with control shRNA (-) (Lane 1) or mTOR shRNA (+)(Lane 2). mTOR was detected using mTOR Antibody BS1844.The mTOR Antibody confirms silencing of mTOR expression.