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Anti-YWHAQ Antibody BHA11300914
Alternative: 14-3-3 protein theta|14-3-3 protein T-cell|14-3-3 protein tau|Protein HS1|YWHAQ|14-3-3 θ|14-3-3 τ|1433T
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: primary antibodies|polyclonal antibodies
Chromosome Location: 2p25.1
Immunogen: Synthetic peptide, corresponding to amino acids 200-240 of Human 14-3-3 θ.
Description: Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.
TARGETWeight: 28 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: 14-3-3 θ (W228) polyclonal antibody detects endogenous levels of 14-3-3 protein theta/tau.
Description: Western blot (WB) analysis of 14-3-3 θ (W228) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug)Lane2:C6 whole cell lysate(40ug)Lane3:NIH-3T3 whole cell lysate(40ug)
Description: Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Description: Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Description: IF image of BS1790 stained A375 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody 14-3-3 θ (W228) pAb #BS1790(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS10950 used at a 1/400 dilution for 2h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).