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Anti-RASGRF1 Antibody BHA11300913
Alternative: Ras-specific guanine nucleotide-releasing factor 1|Ras-GRF1|Guanine nucleotide-releasing protein|GNRP|Ras-specific nucleotide|exchange factor CDC25|RASGRF1|CDC25|GNRP|GRF1
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: Primary Antibodies|Polyclonal Antibodies
Chromosome Location: 15q25.1
Immunogen: Synthetic peptide, corresponding to amino acids 880-932 of Human Ras-GRF1.
Description: A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins.Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.
TARGETWeight: 145 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: Ras-GRF1 (F912) polyclonal antibody detects endogenous levels of Ras-GRF1 protein.
Description: Western blot (WB) analysis of Ras-GRF1 (F912) pAb at 1:500 dilutionLane1:CT26 whole cell lysate(40ug)Lane2:EC9706 whole cell lysate(40ug)Lane3:HEK293 whole cell lysate(40ug)Lane4:PC12 whole cell lysate(40ug)
Description: Immunohistochemistry (IHC) analyzes of Ras-GRF1 (F912) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.