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Anti-ZAP70 Antibody BHA11300763
Alternative: Tyrosine-protein kinase ZAP-70|70 kDa zeta-chain associated protein|Syk-related tyrosine kinase|ZAP70|SRK
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: Primary Antibodies|Polyclonal Antibodies
Chromosome Location: 2q11.2
Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human ZAP70.
Description: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .
TARGETWeight: 70 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: ZAP-70 (T286) polyclonal antibody detects endogenous levels of ZAP-70 protein.