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Anti-NFKB1 Antibody BHA11300419
Alternative: Nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|EBP-1|Nuclear factor of kappa light|polypeptide gene enhancer in B-cells 1|NFKB1|NFkB p105
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: Primary Antibodies|Polyclonal Antibodies
Chromosome Location: 4q24
Immunogen: Synthetic peptide, corresponding to amino acids 920-970 of Human NFkB-p105.
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity
TARGETWeight: 105 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: NFkB-p105 (A901) polyclonal antibody detects endogenous levels of NFκB-p105 protein.This antibody does not recognize NFkB-p50 subunit.
Description: Western blot (WB) analysis of NFkB-p105 (A901) polyclonal antibody at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate(40ug)Lane2:HCT116 whole cell lysate(40ug)Lane3:L02 whole cell lysate(40ug)Lane4:HEK293T whole cell lysate(40ug)
Description: Immunohistochemistry (IHC) analyzes of NFkB-P105 (A901) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Description: Immunoprecipitation of L02 cell lysate using NFkB-p105 (A901) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using NFkB-p105 (A901) pAb #BS1251.“↑”(supernatant); “↓”(deposition)