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Anti-MDM2 Antibody BHA11300393
Alternative: E3 ubiquitin-protein ligase Mdm2|Double minute 2 protein|Hdm2|Oncoprotein Mdm2|p53-binding protein Mdm2|MDM2
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: primary antibodies|polyclonal antibodies
Chromosome Location: 12q15
Immunogen: Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2.
Description: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.
TARGETWeight: 55,90 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: MDM2 (F414) polyclonal antibody detects endogenous levels of MDM2 protein.
Description: Western blot (WB) analysis of MDM2 (F414) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)Lane2:MCF-7 whole cell lysate(40ug)Lane3:H9C2 whole cell lysate(40ug)
Description: Immunohistochemistry (IHC) analyzes of MDM2 (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Description: Western blot (WB) analysis of MDM2 (F414) pAb at 1:500 dilutionLane1:HCC827 whole cell lysate(40ug)Lane2:U-87MG whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug)