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Anti-HSPD1 Antibody BHA11300350
Alternative: 60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|Chaperonin 60|CPN60|Heat shock protein 60|HSP-60|Hsp60|HuCHA60|Mitochondrial matrix protein P1|P60 lymphocyte protein|HSPD1|HSP60
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: Primary Antibodies|Polyclonal Antibodies
Chromosome Location: 2q33.1
Immunogen: Synthetic peptide, corresponding to amino acids 511-560 of Human HSP60.
Description: HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker forr γ/δ T cell recognition.
TARGETWeight: 60 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: HSP60 (T547) polyclonal antibody detects endogenous levels of HSP60 protein.
Description: Western blot (WB) analysis of HSP60 (T547) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate(40ug)Lane2:L02 whole cell lysate(40ug)Lane3:AML-12 whole cell lysate(40ug)Lane4:C6 whole cell lysate(40ug)
Description: Immunohistochemistry (IHC) analyzes of HSP60 (T547) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Description: Immunoprecipitation of L02 cell lysate using HSP60 (T547) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HSP60 (T547) pAb #BS1179.“↑”（supernatant）; “↓”（deposition）