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Anti-ULK1 Antibody BHA11300151
Alternative: Serine/threonine-protein kinase ULK1|Autophagy-related protein 1 homolog|ATG1|Unc-51-like kinase 1|ULK1|KIAA0722
Species: Homo Sapiens (Human)
Delivery: Free shipping. Usually ships in 7 to 10 business days
Categories: Primary Antibodies|Polyclonal Antibodies
Chromosome Location: 12q24.33
Immunogen: Synthetic phosphopeptide derived from human ULK1 around the phosphorylation site of Ser555.
Description: ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.
TARGETWeight: 150 kDa
PROPERTYStorage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.Specificity: ULK1 (phospho-Ser555) polyclonal antibody detects endogenous levels of ULK1 protein only when phosphorylated at Ser555.
Description: Western blot (WB) analysis of PIK3C3/VPS34 (Phospho-Ser249) polyclonal antibody at 1:500 dilutionLane1:LO2 treated with PBS(1×,PH7.4) for 1 hour then treated with DMEM(10%FBS) for 15 minutes whole cell lysateLane2:LO2 treated with PBS(1×,PH7.4) for 1 hour then treated with DMEM(10%FBS) for 5 minutes whole cell lysateLane3:LO2 treated with PBS(1×,PH7.4) for 1 hour whole cell lysateLane4:LO2 whole cell lysate