Anti-MAPK1/MAPK3 Antibody BHA11300102

$199.33
SKU BHA11300102-50µl
Mfr.No.: AP0490

Antigen: MAPK1/MAPK3

Alternative: Mitogen-activated protein kinase 3|MAP kinase 3|MAPK 3|ERT2|Extracellular signal-regulated kinase 1|ERK-1|Insulin-stimulated MAP2 kinase|MAP kinase isoform p44|p44-MAPK|Microtubule-associated protein 2 kinase|p44-ERK1|MAPK3|ERK1|PRKM3|Mitogen-activated protein kinase 1|MAP kinase 1|MAPK 1|ERT1|Extracellular signal-regulated kinase 2|ERK-2|MAP kinase isoform p42|p42-MAPK|Mitogen-activated protein kinase 2|MAP kinase 2|MAPK 2|MAPK1|ERK2|PRKM1|PRKM2

Reactivity: Human|Mouse|Rat

Host: Rabbit

Clonality: Polyclonal

Application: WB|IHC|IF

Vendor: bioworld

Recombinant: FALSE

Regulatory: RUO

Delivery: Free shipping. Usually ships in 7 to 10 business days

Categories: Primary Antibodies|Polyclonal Antibodies

Immunogen: Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.

Modification: phospho-Y204

Description: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Properties

TARGET

Weight: 42,44 kDa

ORGANISM

Swissprot: P27361/P28482

Uniprot: P27361/P28482

PROPERTY

Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Desc: Product: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.Specificity: p-ERK1/2 (Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at Tyr204, and ERK2 protein when phosphorylated at Tyr187.
Protocols
Datasheet:
Applications
Apps: WB|IHC|IF

Dilution:

WB 1:500-1:1000
IHC 1:50-1:200
IF 1:50-1:200
Images


Description: Western blot (WB) analysis of p-ERK1/2 (Y204) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate treated with UV(4h)Lane2:NIH-3T3 whole cell lysate treated with UV(4h)Lane3:PC12 whole cell lysate treated with UV(4h)


Description: LO2 cell, treated with LPS(1000ng/ml) for 15 miniutes, untreated or λ phosphatase-treated.The western blot was probed using p-ERK1/2 (Y204) pAb #AP0490 at 1:500 dilution.

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