Anti-Trigger factor Polyclonal Antibody

What it means: Polyclonal antibodies are a mixture of immunoglobulins from many B-cell lineages that together recognize multiple epitopes on the same target — here bacterial Trigger factor.

Why it matters:

• Multi-epitope binding gives Polyclonal reagents strong signal amplification, useful for detecting moderately expressed bacterial proteins by WB or IHC.
• Epitope redundancy makes detection tolerant of partial denaturation and fixation effects.

How to interpret here: This Polyclonal was raised against nearly full-length Trigger factor (Met1-Ala432), so epitope coverage across the protein underlies its broad reactivity over thirty-nine enterobacterial strains.

Tip: For long-term standardization needs, consider pairing with a monoclonal once your assay is established.

What it means: The host is the species immunized to produce the antiserum — this antibody is Rabbit-derived IgG purified by antigen affinity chromatography.

Why it matters:

• Detection requires an anti-Rabbit IgG secondary antibody in WB, IHC, or ELISA.
• Rabbit polyclonals typically offer high affinity and robust performance across buffer conditions.

How to interpret here: Because the targets are bacterial, a Rabbit host means essentially no background concern from your bacterial samples themselves; standard anti-rabbit HRP or fluorescent secondaries work directly.

Tip: When staining mammalian tissue infected with bacteria by IHC, include a no-primary control to account for endogenous immunoglobulin binding.

What it means: Trigger factor is the ribosome-associated chaperone of bacteria — a ~48 kDa protein (gene tig) that binds nascent chains at the ribosomal exit tunnel and also acts as a peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8).

Why it matters:

• Trigger factor is a central model protein for co-translational folding and chaperone-network studies.
• It is also monitored as a host-cell protein and solubility-tag component in recombinant expression workflows.

How to interpret here: This antibody detects Trigger factor across E. coli, Salmonella, Shigella, and related genera thanks to strong sequence conservation.

Tip: Expect a band near 48–56 kDa on WB depending on gel system.

What it means: The validated applications for this antibody are ELISA, Immunohistochemistry, Western Blot — each with a vendor-provided dilution window.

Why it matters:

• WB validation (1:500–1:2000) confirms specific detection of denatured Trigger factor in lysates.
• IHC validation (1:50–1:500) enables in-situ localization of bacteria in tissue sections; ELISA (1:5000–1:20000) supports quantitative work.

How to interpret here: Start at the middle of each range — e.g. 1:1000 for Western Blot — and titrate to your sample type. Because ELISA, Immunohistochemistry, Western Blot span both native and denatured antigen formats, this coverage indicates the antibody recognizes linear epitopes that survive SDS treatment as well as fixed-tissue presentation.

Tip: Validation does not transfer between applications; re-optimize when you switch formats.

What it means: IgG is the immunoglobulin class of this antibody — the standard, most stable antibody format for research reagents, purified here from rabbit antiserum.

Why it matters:

• Secondary antibodies, protein A/G capture media, and most conjugation kits are optimized for IgG.
• IgG stability supports the 50% glycerol liquid format and routine freezer storage.

How to interpret here: Use any anti-rabbit IgG secondary; for isotype-control experiments, choose a non-immune rabbit IgG at matched concentration. Since this IgG is antigen affinity purified rather than total serum IgG, the specific-antibody fraction is high, so lower working concentrations are often sufficient compared with crude antiserum.

Tip: Record the isotype in your methods so readers can reproduce the detection chain exactly.

What it means: Bacteria reactivity indicates the antigen recognized by this antibody is a bacterial protein — Trigger factor expressed by enteric bacteria — rather than a mammalian host protein or viral antigen.

Why it matters:

• For antibodies against Bacteria, strain and serotype coverage — not host species — is the compatibility question to check before ordering.
• Trigger factor is highly conserved across Enterobacteriaceae, which is what gives this reagent its unusually broad thirty-nine-strain reactivity list.

How to interpret here: The detailed list spans Escherichia coli (multiple serotypes), Salmonella, Shigella, Klebsiella pneumoniae, Cronobacter sakazakii, Citrobacter koseri, and Enterobacter species — confirm your specific strain or serotype appears there.

Tip: For unlisted but related species, sequence-align the tig gene product before assuming cross-reactivity.