Matrices listed below were spiked with certain level of GSK3beta and the recovery rates were calculated by comparing the measured value to the expected amount of GSK3beta in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
99
EDTA plasma(n=5)
86-103
97
heparin plasma(n=5)
88-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSK3beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GSK3beta ELISA Kit allows for the in vitro quantitative determination of GSK3beta concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat GSK3beta(Glycogen synthase kinase-3 beta) ELISA Kit