Interleukin 17D is a disulfide-linked homodimer belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL17 family is inducible by IL15 and IL23, and IL17D induces expression of endothelial IL6, IL8, and GM-CSF, and inhibits hemopoiesis of myeloid progenitor cells.
Alternative Names
Interleukin-27
Quantity
1.0 mg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
The ED50 is 7.5-37.5 ng/mL as determined by its binding ability in a functional ELISA (at a concentration of 1 µg/mL)
Weight
20.5 kDa
Description
Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
Format
Lyophilized PowderLyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.