Human deubiquitinating enzyme ovarian tumor domain-containing 6B, or OTUD6B (GenBank Accession No. NM_016023), a.a. 2-323 (end) with N-terminal His-FLAG-tag, MW=39 kDa, expressed in E. coli cells.
Specific activity: 24 pmole/min/mg
Immunogen Region
2-323 (end)
Tag
N-terminal His-FLAG-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT. 0.5 mM EDTA, 500 nM Ub-AMC and OTUD6B. Reaction was incubated at 37°C for 15 min and fluorescent signal was measured at excitation of 340 nm and emission at 460 nm.
Avoid freeze/thaw cycles.
GenBank
NM_010623
Mw(kda)
39 kDa
Synonyms
DUB, OTUD6B, ovarian tumor domain
Uniprot
Q8N6M0
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Xu, Z. et al., PLoS One. 2011 Jan 18; (6)1: e14514. 2. Sowa, ME. et al., Cell. 2009 Jul 23; 138(2): 389-403.