Matrices listed below were spiked with certain level of IFNalpha4 and the recovery rates were calculated by comparing the measured value to the expected amount of IFNalpha4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
93
EDTA plasma(n=5)
88-95
91
heparin plasma(n=5)
88-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNalpha4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-105%
87-99%
90-97%
EDTA plasma(n=5)
84-98%
83-101%
86-97%
heparin plasma(n=5)
84-100%
80-92%
83-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P05014
Applications
ELISA
Applications Notes
IFNalpha4ELISA Kit allows for the in vitro quantitative determination of IFNalpha4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IFNalpha4(Interferon alpha-4)ELISA Kit