Human CD25(Interleukin 2 Receptor Alpha) ELISA Kit

Mfr.No.

EH0081

Alternative Names

CD25 ELISA Kit, IL-2Ralpha ELISA Kit, Interleukin 2 Receptor Alpha ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

93.75-6000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of CD25 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-99	94
EDTA plasma(n=5)	86-104	95
heparin plasma(n=5)	85-98	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-101%	91-103%	87-105%
EDTA plasma(n=5)	83-97%	82-101%	83-97%
heparin plasma(n=5)	82-99%	83-95%	83-94%

Sensitivity

< 56.25pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

CD25/IL-2Rα/Interleukin 2 Receptor Alpha

Notes

For Research Use Only

UniProt ID	P01589
Antigen	CD25

Applications	ELISA
Applications Notes	CD25 ELISA Kit allows for the in vitro quantitative determination of CD25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Cancer Communications Title: IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805477	EH0081	96T	$400		Add to Cart

Human CD25(Interleukin 2 Receptor Alpha) ELISA Kit

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