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Home / Antibodies

Ubiquitin-conjugating enzyme E2 B Antibody / UBE2B (V9247)

SKU : BHA17119635

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NSJ Bioreagents

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Mfr.No.	V9247, V9247-20UG, V9247-100UG
Clonality	Monoclonal (mouse origin)
Description	The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Recombinant full-length human UBE2B protein was used as the immunogen for the UBE2B antibody.
Isotype	Mouse IgG2b
Species Reactivity	Human
Note	Optimal dilution of the UBE2B antibody should be determined by the researcher.

Uniprot	P63146

Format	Purified
Purity	Protein A/G affinity
Storage	Aliquot the UBE2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Applications	FACS, IF
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml

Product Manual	Datasheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17119635-1	V9247-20UG	20ug	$219		Add to Cart
BHA17119635-2	V9247-100UG	100ug	$499		Add to Cart

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