This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA DOPA to DOPA-quinone and possibly 56-dihydroxyindole to indole-56 quinone.Patricia Giraldo Nucleic Acids Res. Nov 2003; 31: 6290 - 6305. R Halaban J. Cell Biol. Aug 1983; 97: 480. Hee-Young Park J. Biol. Chem. Jun 1999; 274: 16470. BB Fuller J. Biol. Chem. Mar 1987; 262: 4024 - 4033.
Host
Rabbit
Immunogen
Synthesized peptide derived from Internal of Human tyrosinase.
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
Patricia Giraldo, Nucleic Acids Res., Nov 2003; 31: 6290 - 6305. R Halaban, J. Cell Biol., Aug 1983; 97: 480. Hee-Young Park, J. Biol. Chem., Jun 1999; 274: 16470. BB Fuller, J. Biol. Chem., Mar 1987; 262: 4024 - 4033.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total tyrosinase protein.
Subcellular Location
Melanosome membrane, Single-pass type I membrane protein, Melanosome
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Pathway
Melanogenesis
Protein Families
Tyrosinase family
Buffer
pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.al
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
Liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
WB
Description
WB
Dilution
WB
1:500-3000
Tested Application
WB Recommended dilution: Western blotting: 1:500~1:3000